Publikationen
Isolierung seltener Einzelzellen: zirkulierende Tumorzellen (CTCs)
- Ding P.N. et al. The predictive and prognostic significance of liquid biopsy in advanced epidermal growth factor receptor-mutated non-small cell lung cancer: A prospective study. Lung Cancer 134:42-45 (2019)
- Sprouse M.L. et al. PMN-MDSCs Enhance CTC Metastatic Properties through Reciprocal Interactions via ROS/Notch/Nodal Signaling. Int. J. Mol. Sci. 20(8):1916, (2019)
- Bang-Christensenet S.R. et al. Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein. Cells 8(9):998, (2019)
- Reinhardt, F. et al. Diagnostic Leukapheresis Enables Reliable Transcriptomic Profiling of Single Circulating Tumor Cells to Characterize Inter-Cellular Heterogeneity in Terms of Endocrine Resistance. Cancers (Basel), 11(7), (2019)
- Szczerba, B. et al. Neutrophils escort circulating tumour cells to enable cell cycle progression Nature 566: 553-557 (2019)
- Donato C. et al. Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment. J. Vis. Exp. (147), e59677 (2019)
- Gkountela, S. et al. Circulating Tumor Cell Clustering Shapes DNA Methylation to Enable Metastasis Seeding Cell 176 (1-2): 98-112.e14 (2019)
- Cabezas-Camarero, S. et al. RAS analysis of circulating tumor cells from advanced colorectal cancer using BEAMing technology. Journal of Clinical Oncology 37 (15_suppl) (2019)
- Kim, D.U. et al. Comprehensive characterization of single circulating rare cells through automated picking and high-throughput mRNA profiling in patients with pancreatic cancer. Journal of Clinical Oncology, 37 (4_suppl) (2019)
- Nelep, C. et al. Automated rare single cell picking with the ALS CellCelector Cytometry Part A, cyto.a.23568. [Epub ahead of print] (2018)
- Agerbæk, M.Ø. et al. The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner Nature Commun. 9(1):3279 (2018)
- Krol, I. et al. Detection of circulating tumour cell clusters in human glioblastoma British Journal of Cancer 119:487-491 (2018)
- Lampignano, R. et al. A Novel Workflow to Enrich and Isolate Patient-Matched EpCAMhigh and EpCAMlow/negative CTCs Enables the Comparative Characterization of the PIK3CA Status in Metastatic Breast Cancer Int. J. Mol. Sci. 18, 1885 (2017)
- Giannopoulou, L. et al. Liquid biopsy in ovarian cancer: recent advances on circulating tumor cells and circulating tumor DNA (Review) Clin. Chem. Lab. Med. Epub (2017)
- Ma, Y. et al. Droplet Digital PCR Based Androgen Receptor Variant 7 (AR-V7) Detection from Prostate Cancer Patient Blood Biopsies Int. J. Mol. Sci. 17(8): E1264 (2016)
- Neumann, M.H. et al. Isolation and characterization of circulation tumor cells using a novel workflow combining CellSearch® and CellCelectorTM Biotechnol Prog. 33(1): 125-132 (2016)
- Blassl, C. et al. Gene expression profiling of single circulating tumor cells in ovarian cancer - Establishment of a multi-marker gene panel Molecular Oncology 10(7): 1030-42 (2016)
- Schneck, H. et al. EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer PLoS One 10(12): e0144535 (2015)
- Lohr, G.J. et al. Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer Nature Biotechnology 32: 479-484 (2014)
- Yao, X. et al. Tumor cells are dislodged into the pulmonary vein during lobectomy J. Thorac Cardiovasc. Surg. 148(6): 3224-31 (2014)
- Heidary, M. et al. The dynamic range of circulating tumor DNA in metastatic breast cancer Breast Cancer Res. 16(4): 421 (2014)
- Adalsteinsson, V.A. et al. Single cells from human primary colorectal tumors exhibit polyfunctional heterogeneity in secretions of ELR + CXC chemokines Integr. Biol. (Camb.) 5(10): 1272-81 (2013)
- Lampignano, R. et al. The combination of ParsortixTM and CellCelectorTM enables the characterisation of EpCAMneg CTCs in breast cancer Poster ISMRC 2016 Hamburg
- Neumann, M. et al. CellCelector isolation of CTCs enables additional marker staining followed by panel sequencing Poster ISMRC 2016 Hamburg
- Neumann, M. et al. CellCelector and CellSearch combined workflow Poster ACTC 2014 Crete
- Voigt, K. et al. Single cell sequencing of rare cells after magnetic enrichment Poster ISRMC 2013 Paris
- Berger, T. et al. Detection and characterization of CTCs in patients with hepatocellular carcinoma (HCC) Poster DGHO 2013 Vienna
Isolierung seltener Einzelzellen: fetale Zellen (zellbasierte NIPT)
- Vestergaard E.M. et al. - On the road to replacing invasive testing with cell‐based NIPT: Five clinical cases with aneuploidies, microduplication, unbalanced structural rearrangement, or mosaicism. Prenatal Diagnostics 37(11):1120-1124 (2017)
- Kølvraa, S. et al. Genome-wide copy number analysis on DNA from fetal cells isolated from the blood of pregnant women Prenatal Diagnostics 36: 1-8 (2016)
Einzelzellisolierung und Analyse
- Baron, C.S. et al. Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in aorta Nature Commun. 9(1): 2517 (2018)
- Wang, J. et al. Single cell sequencing: a distinct new field [Review] Clin. Trans. Med. 6(1): 10 Epub Feb 20 (2017)
- Ku, C.J. et al. GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion Mol Cell Biol. Epub Mar 20 (2017)
- Dura, B. et al. Longitudinal multiparameter assay of lymphocyte interactions from onset by microfluidic cell pairing and culture PNAS 113(26): E3599-608 (2016)
- Liao, M.C. et al. Single-Cell Detection of Secreted Aß and sAPPa from Human IPSC-Derived Neurons and Astrocytes J. Neurosci. 36(5): 1730-46 (2016)
- Wang, Y. et al. Advances and applications of single-cell sequencing technologies (Review) Molecular Cell. 58(4): 598-609 (2015)
- Castellarnau, M. et al. Stochastic Particle Barcoding for Single-Cell Tracking and Multiparametric Analysis Small 11(4): 489-498 (2015)
- Navin, N.E. Cancer genomics: one cell at a time (Review) Genome Biol. 15(8): 452 (2014)
- Cornils, K. et al. Multiplexing clonality - combining RGB marking and genetic barcoding Nucl. Acid Res. 42(7): e56 (2014)
- Wuethrich, I. et al. A Mouse Monoclonal Antibody Against Alexa Fluor 647 Monoclonal Antibodies in Immunodiagnosis and Immuntherapy 33(2): 109-120 (2014)
- Kluth, S.M. et al. Increased Haematopoietic Supportive Function of USSC from Umbilical Cord Blood Compared to CB MSC and Possible Role of DLK-1 Stem Cells Int. 985285 (2013)
- Sendra, V.G. et al. Detection and isolation of auto-reactive human antibodies from primary B cells Methods 64(2): 153-9 (2013)
- Varadarajan, N. et al. High-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis J. Clin. Invest. 121(11): 4322-31 (2011)
- Choi, J.H. et al. Development and optimization of a process for automated recovery of single cells identified by microengraving Biotechnol. Prog. 26(3): 888-95 (2010)
- Zoldan, K. et al. Visualizing of migration, interaction, proliferation or differentation of cells in time lapse exposures using the cell separation robot CellCelectorTM nature methods application notes 2010
- Wagner, K. et al. Selection of reference genes for quantitative PCR of single cells Poster STS 2011
Isolierung adhärenter Kolonien (Stammzellen etc.)
- Segerman, A. et al. Clonal Variation in Drug and Radiation Response among Glioma-Initiating Cells Is Linked to Proneural-Mesenchymal Transition Cell Reports 17: 2994-3009 (2016)
- Cohen, I.S. et al. DNA lesion identity drives choice of damage tolerance pathway in murine cell chromosomes Nucl. Acids Res. 43(3): 1637-45 (2015)
- Shipony, Z. et al. Dynamic and static maintenance of epigenetic memory in pluripotent and somatic cells Nature 513(7516): 115-9 (2014)
- Marx, U. et al. Automatic Production of Induced Pluripotent Stem Cells Procedia CIRP 5: 2-6 (2013)
- Haupt, S. et al. Automated selection and harvesting of pluripotent stem cell colonies Biotechnol. Appl. Biochem. 59(2): 77-87 (2012)
- Zoldan, K. et al. Automated harvest of induced pluripotent stem cell colonies and colony fractions using the cell separation robot CellCelectorTM nature methods application notes (2010)
- Zoldan, K. et al. Automated isolation of semi-adherent macrophage-like cells from a fibroblast-contaminated culture using the cell separation robot CellcelectorTM nature methods application notes (2010)
- Haupt, S. et al. Automated selection and harvesting of pluripotent stem cell colonies using the CellCelector Nature Methods (2009)
- Schneider, A. et al. "The good into the pot, the bad into the crop!" - a new technology to free stem cells from feeder cells PLoS One 3(11): e3788 (2008)
- Peterbauer, T. et al. Simple and versatile methods for fabrication of arrays of live mammalian cells Lab on a Chip 6(7): 857-63 (2006)
- Planes, E. et al. Life Cell Imaging for Quality Control of Pluripotent Stem Cell Culture Poster ISSCR 2013 Boston
Picken von Klonen aus halbfesten Medien
- Vaniotis, G. et al. Enhanced anti-metastatic bioactivity of an IGF-TRAP re-engineered to improve physicochemical properties Scientific Reports 8: 17361 (2018)
- Zoldan, K. et al. Automated clonal selection of high-producing hybridoma colonies from methylcellulose-based, semi-solid medium using the cell separation robot CellCelectorTM nature methods application notes (2010)
- Caron A.W. et al. Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins (CHO) BMC Biotechnol. 9: 42 (2009)
- Julien, A. et al. Antigen specific antibody-producing clones selected by isotypes Biosystems International Application Note
- Gilbert, R. Fluorescent labeling in Semi-Solid Medium to Identify High Expressing Clones for Recombinant Protein Production (CHO) CHI's Bioprocessing Summit Presentation, Boston 2011